The influence of external irradiation and cryopreservation on sperm quality of stud bulls

«Radiation and Risk», 2014, vol. 23, No. 3, pp.26-33

Authors

Astasheva N.P.1 – Prof., Dr. Sci., Biol., Financial Technological Academy, Korolyov, Russia. Contacts: 42 Gagarin St., Korolyov, Moscow region, Russia, 141075. Tel.: (905) 55-47-156; е-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Lazarev N.M.2 – Head of Lab., C. Sc., Biol., Institute of Agricultural Radiology, National University of Bioresources and Environmental Management of Ukraine, Kyiv, Ukraine.

Abstract

The purpose of the study is to investigate combined effect of external radiation exposure and cryopreservation on the semen quality of fertile bulls. The study presents results of research on clinical and physiological state of stud bulls exposed to sublethal doses of external radiation and effect of cryopreservation on the semen quality. The bulls divided in 4 groups, the animals of each group were exposed to the single radiation dose of 1, 2, 3, and 4 Gy. Blood was tested in 3, 7, 15, 30, 45, 60 days after exposure. Sperm was taken every week, after testing its quality the sperm was frozen and kept in liquid nitrogen. On the 7th day after the exposure of 3 Gy and 4 Gy the concentration of white blood cells decreased by 44% and 55% respectively, as compared to the initial level. Authors present dynamic data on dead sperm cells level in the cryopreserved and thawed sperm in relation to a radiation dose received by the bulls. They demonstrate the dependence of the semen quality on dose. As compared with the control points, concentration of sperm decreased by 40-75% in 8 weeks after the exposure of the bulls to doses of 1-4 Gy, there was a significant decline in motility and viability. Sperm motility recovered in 38-40 weeks after exposure to radiation.

Key words
external radiation exposure, fertile bulls, sublethal radiation dose, sperm cryopreservation, blood test, thawing frozen semen, semen quality.

References

1. Konoplya Ye.F., Vereshchako G.G., Khodosovskaya A.M., Rybakov V.N. Morfofunktsional'noye sos-toyaniye reproduktivnoy sistemy krys-samtsov posle khronicheskogo nizkointensivnogo oblucheniya v doze 1,0 Gr [Morphofunctional-channel state of the reproductive system of male rats after chronic low-level exposure to a dose of 1.0 Gy]. Radiatsionnaia biologiia. Radioekologiia – Radiation Biology. Radioecology, 2002, vol. 42, no. 2, pp. 136-140.

2. Astasheva N.P., Frunze V.Ya. Vliyanie razlichnykh doz oblucheniya na vosproizvoditel'nuyu funktsiyu baranov [Influence of various doses of radiation on reproductive function of rams]. Problemy sel'skokho-zyaystvennoy radiologii: sbornik nauchnykh trudov – Problems of agricultural radiology. Collection of scientific works. Kiev, 1993, pp. 179-184.

3. Novikova O.N. Otsenka kachestva spermy khryakov, podvergshikhsya odnokratnomu total'nomu gamma-oblucheniyu: avtoref. dis. … kand. biol. nauk [Quality assessment of pig sperm following single whole-body gamma-radiation exposure. Cand. biol. sci. diss. synopsis]. Dubrovitsa, PMP VIZh, 1991. 28 p.

4. GOST 27777-88. Sperma bykov zamorozhennaja. Metody biologicheskih issledovani.j [State Standard 27777-88. Frozen sperm of bulls. Methods of biological test]. Moscow, Standartinform Publ., 1989. 7 p.

5. Jushhenko N.P. Zamorazhivanie spermy zhivotnyh v granulah zhidkim azotom [Freezing of granulated animal sperm in liquid nitrogen]. Vosproizvodstvo i iskusstvennoe osemenenie sel'skohozjajstvennyh zhivotnyh [Reproduction and artificial insemination of agricultural animals]. VASKhNIL – Lenin All-Union academy of Agricultural Sciences, 1990, pp. 31-38.

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