Conditioning activities of the second generation of male rats exposed to acute gamma-radiation of 1.0 Gy. Part 2

«Radiation and Risk», 2014, vol. 23, No. 3, pp.80-88

Authors

Panfilova V.V. – Postgraduate Student, Medical Radiological Research Center, Ministry of Health of the Russian Federation, Obninsk, Russia. Contacts: 4 Korolyov str., Obninsk, Kaluga region, Russia, 249036. Tel.: (484) 399-71-38; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Kolganova O.I. – Senior Researcher, C. Sc., Biol., Medical Radiological Research Center, Ministry of Health of the Russian Federation, Obninsk, Russia.
Zhavoronkov L.P. – Deputy Director, MD, Medical Radiological Research Center, Ministry of Health of the Russian Federation, Obninsk, Russia.
Pavlova L.N. – Lead. Researcher, C. Sc., Med., Medical Radiological Research Center, Ministry of Health of the Russian Federation, Obninsk, Russia.
Chibisova O.F. – Researcher, Medical Radiological Research Center, Ministry of Health of the Russian Federation, Obninsk, Russia.

Abstract

Available experimental data do not allow us to state unambiguously about transmission of radiation-induced impairments of higher nervous activity from irradiated parents to their offspring. The purpose of the study is to elucidate an effect of whole-body gamma-irradiation of Wistar male rats at dose of 1.0 Gy on psycho-physiological development of the next generations. The following methods of crossing were used: 1) females of the first generation were mated with nonirradiated males, their offspring were the second generation, F2, on the maternal line; 2) males of the first generation were mated with nonirradiated females, their offspring were the second generation, F2, on the paternal line; 3) females of the first generation were mated with males of the first generation, the offspring were the second generation, F2, their grandfathers both on maternal and paternal lines were exposed to radiation. Cognitive function in F2 offspring was tested using shuttle-box avoidance method. The authors found that heritable impairments transmitted from the F1 generation occurred more often in the F2 generation of the paternal line than on the maternal line. If both parents of the first generation were offspring of irradiated males, in this case the impairments transmitted from F1 to F2 generation were amplified.

Key words
Ionizing radiation, dose of 1.0 Gy, Wistar rats, second generation, paternal line, maternal line, brain cognitive functions, conditioned avoidance reflex, Shuttle-box, psychophysiological development, spermatogenesis.

References

Baleva L.S. Opredelenie grupp riska vozniknoveniya radiatsionno-indutsirovannoy i somaticheskoy patologii u detey, podvergshikhsya vozdeystviyu ra-diatsii, v tom chisle potomkov pervogo-vtorogo pokoleniy, rozhdennykh u obluchennykh roditeley, i obosnovanie neobkhodimoy meditsinskoy pomoshchi na etapakh nablyudeniya [Identification of groups at risk of radiation induced abnormalities and somatic diseases in children exposed to radiation, as well as children and grandchildren of people exposed to ionizing radiation. Reasoning for delivery of necessary health care during the follow-up]. Moscow, ed.: Baleva L.S., Textbook for Doctors, 2007. 19 p.

2. Ermalitskiy A.P. Obespechenie effektivnosti radiatsionno-gigienicheskikh mer pri zashchite reproduktivnogo zdorov'ya muzhchin, rabotayushchikh v kontakte s ionizi-ruyushchim izlucheniem: Avtoref. diss. kand. med. nauk. [Assurance of due performance of measures to protect reproductive health of men working with ionizing radiation. Cand. med. sci. diss. synopsis]. Moscow, Burnazyan Research Centre Publ., 2012. 29 p.

3. Panfilova V.V., Kolganova O.I., Zhavoronkov L.P., Pavlova L.N., Ivanov V.L., Palyga G.F. Uslovno-reflektornaia deiatelnost potomstva pervogo pokolenia ot samtsov krys, podvergshisia ostromu gamma oblucheniu v doze 1,5 Gr [Conditioning activities of the first generation of male rats exposed to acute gamma radiation of 1.0 Gy. Part 1]. Radiatsiya i risk – Radiation and Risk, 2014, vol. 23, no. 3, pp. 70-79.

4. Nefedov I.Yu. Nasledstvennye posledstviya oblucheniya oboikh roditeley (eks-perimental'noe issledovanie na krysakh linii Wistar). Avtoref. diss. doct. biol. nauk. [Hereditary effects of exposure of both parents to ionizing radiation. Experimental study in Wistar rats. Doct. biol. sci. diss. synopsis]. Obninsk, 1998. 52 p.

5. Koterov A.N., Biryukov A.P. Deti uchastnikov likvidatsii posledstviy avarii na Chernobyl'skoy atomnoy stantsii. Soobshchenie 1. Otsenka printsipial'noy vozmozh-nosti zaregistrirovat' radiatsionnye effekty (Obzor) [Offspring of participants of clean-up operations following the accident at the Chernobyl NPP. Part 1. Assessment of feasibility to detect radiation-induced changes. Review]. Meditsinskaya radiologiya i radiatsionnaya bezopasnost' – Medical Radiology and Radiation Safety, 2012, vol. 57, no. 1, pp. 58-79.

6. Pavlova L.N., Zhavoronkov L.P., Palyga G.F., Kolganova O.I., Glushakova V.S., Chibisova O.F., Ivanov V.L. Psikhofiziologicheskoe razvitie dvukh pokoleniy potomstva samok krys, khronicheski obluchennykh v doze 1 Gr vo vremya beremennosti, i vozmozhnost' ego modifikatsii s pomoshch'yu melanina [Psychophysiological development of two generations of female rats chronically exposed to gamma-irradiation during pregnancy in the total dose of 1 Gy and its modification by melanin]. Radiatsionnaya bi-ologiya. Radioekologiya – Radiation Biology. Radioecology, 2007, vol. 47, no. 2, pp. 171-180.

Full-text article (in Russian)