Applicability of gamma-radiation for obtaining test-culture Bacillus Subtilis in measurement of beta-lysine activity in the blood

«Radiation and Risk», 2016, vol. 25, No. 1, pp.108-114

Authors

Saruhanov V.Ya. – Senior Researcher, C. Sc., Biol. All-Russian Scientific Research Institute of Radiology and Agroecology, Obninsk, Russia. Contacts: Kievskoe Sh., 109 km, Obninsk, Kaluga region, Russia, 249032. Tel.: 89056402821; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. . RSRIRA.
Kolganov I.M.¹ – Head of Lab., C. Sc., Tech. Research and Production Enterprise «Eco-filter», Obninsk, Russia.
Tsarin P.G.¹ – General Director. Tech. Research and Production Enterprise «Eco-filter», Obninsk, Russia.

Abstract

β-lysine activity in the blood is one of informative indicators of physiological status of a human and animals, that is why measuring its level is very important for application a test in medicine and veterinary. However, the use of the test is limited because the reaction is a labor-consuming process. The purpose of the study is examining the applicability of γ-radiation for obtaining test-culture Bacillus subtilis of optimal size for quantitative measurement of β-lysine activity in the blood of farm animals and a human. The level of β-lysine activity in the blood of sheeps, horses and a human measured with native and γ-irradiated suspension was reliably similar. Comparative analysis of seasonal changes of β-lysine activity in the blood of cows measured with native and irradiated testcultures Bacillus subtilis confirmed general common biological regularity that the highest level of β-lysine activity is in autumn winter season, and the lowest level is in the spring-summer season. The results of the study show that the use of γ-radiation for obtaining test-culture Bacillus subtilis with optimal size of particles without preliminary filtration allows to simplify the measuring β-lysine activity in the blood of farm animals and a human, as well as to reduce significantly consumption of reagents.

Key words
Farm animals, laboratory animals, natural resistance, γ-radiation, test-culture, Bacillus subtilis, microbial aggregates, β-lysine activity, blood serum, blood plasma.

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