Effects of ionizing radiation and feeding restriction on physiological, clinical, biochemical parameters and fertility of heifers (experimental research)

«Radiation and Risk», 2017, vol. 26, No. 4, pp.132-144

DOI: 10.21870/0131-3878-2017-26-4-132-144

Authors

Astasheva N.P.1 – Prof., D.Sc., Biol. Technological University, Korolyov. Contacts: 42 Gagarin St., Korolyov, Moscow region, Russia, 141075. Tel.: 8(905)5547156; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Ulyanenko L.N.2 – Lead. Res., Prof., D.Sc., Biol. A. Tsyb MRRC, Obninsk.

Abstract

Chernobyl accident, its effects on human health, environment, agriculture and farm livestock set up a problem of preparedness and response to radiation emergency for veterinarians. To identify measures for preparedness and mitigating adverse effects of radiation on livestock we should know response of animals to external and internal radiation, as well as effects of confounding factors. The article presents results of the experiment aimed to describe combined effect of external γ-radiation and feeding level on health status, physiological and biochemical parameters and fertility in heifers. Twenty two heifers of black-and-white breed (age 14-18 months) were exposed to sublethal doses of external gamma-radiation (2.5 и 3.25 Gy), in postradiation period the scenario modelling the worst post-emergency housing and maintenance was applied: 4-day hunger, 1 month   feed restrictions. Health and physiological status as well as the dynamic of the biochemical parameters of blood and reproductive capacity were estimated. On the 3rd day after radiation exposure and hunger the body weight of the heifers decreased by 22-28 kg, p<0.05. In the animals exposed to 3.25 Gy, not experiencing hunger, no changes in the body weight were found. The most decrease in white blood cells (WBC) count was found in the animals exposed to 3.25 Gy and experienced hunger on the 3rd day following irradiation. On the 7th day after exposure of the animals experiencing feed restrictions to 3.25 Gy the WBC count decreased by 5 times. Changes in such biochemical parameters as total protein content, alkaline phosphatase activity and alpha-amylase activity in the blood plasma were of wave-like nature due to severity of postradiation health disorder, different phases of radiation damage, restricted feeding regime and maintenance. During the follow-up period we observed the largest changes in biochemical parameters in the group of heifers experienced feed restrictions and exposed to 3.25 Gy. In the animals exposed to 2.5 Gy and experienced feed restrictions biochemical parameters were close to the parameters in the heifers exposed to 3.25 Gy and fed standard diet. Exposure to external gamma-radiation to 2.5 and 3.25 Gy and temporary diet restrictions did not cause any changes in the regularity and manifestation of sexual cycles. All animals exposed to radiation dose of 3.25 Gy and fed standard diet gave birth to their calves, 60% of animals exposed to 3.25 Gy and experienced feeding restrictions gave birth to calves. Health status of calves in both groups calves of both group, similar. Investigations with animals were carried out in accordance with the basic requirements of the Russian national standard GOST 33215-2014.

Key words
External gamma-irradiation, change technology content, feeding restriction, sexually mature heifers, health and physiological status, biochemical parameters, reproductive capacity.

References

1. Polyantsev N.I. Vosproizvodstvo v promyshlennom zhivotnovodstve [Reproduction in the livestock industry]. Moscow, Rosagropromizdat Publ., 2012. 240 p.

2. Semivolos A.M. Veterinarnoe akusherstvo i biotekhnika reproduktsii zhivotnykh [Veterinary obstetrics and bioengineering of animal reproduction]. Saratov, «Saratovi GAU» Publ., 2014. 93 p.

3. Masalov V.N. Zavisimost' reproduktivnoy funktsii cherno-pestrykh golshtinizirovannykh korov ot raznykh faktor [The dependence of reproductive function black-and-white holsteinized cows of different factors]. Zootehniya – Husbandry, 2007, no. 4, pp. 25-27.

4. Onozheev A.A. Obshchaya patologiya: uchebnoe posobie. [General pathology: textbook]. Ulan-Ude, Publ. BGSHA im. V.R. Filippova, 2009. 136 p.

5. Vayserman A.M., Koshel' N.M., Mekhova L.V., Voytenko V.P. Radiatsionnyy gormezis v eksperimental'nykh issledovaniyakh [Radiation hormesis in experimental studies]. Meditsinskaya radiologiya i radiatsionnaya bezopasnost't – Medical Radiology and Radiation Safety, 2011, vol. 56, no. 4, pp. 5-16.

6. Konoplya E.F., Vereshchako G.G., Khodosovskaya A.M., Rybakov V.N. Morfofunktsional'noe sostoyanie reproduktivnoy sistemy krys-samtsov posle khronicheskogo nizkointensivnogo oblucheniya v doze 1,0 Gr [Morphofunctional-channel state of the reproductive system of male rats after chronic low-level exposure to a dose of 1.0 Gy]. Radiatsionnaya biologiya. Radioekologiya – Radiation Biology. Radioecology, 2002, vol. 42, no. 2. pp. 136-140.

7. Astasheva N.P., Lazarev N.M. Vliyanie vneshnego oblucheniya i kriokonservatsii na kachestvo spermy bykov-proizvoditeley [The influence of external irradiation and cryopreservation on sperm quality cattle pro-ducers]. Radiatsiya i risk – Radiation and Risk, 2014, vol. 23, no. 3, pp. 26-33.

8. Isamov N.N., Isamov N.N. Jr. Radiobiologiya i radioekologiya loshadey [Radiobiology and radioecology of horses]. Ed.: R.M. Aleksakhin. Obninsk, RIARAE Publ., 2009. 112 p.

9. Normy i ratsiony kormleniya sel'skokhozyaystvennykh zhivotnykh. Spravochnoe posobie. 3 izdanie. [Norms and rations of feeding of agricultural animals. A reference guide]. Eds.: A.P. Kalashnikov, V.I. Fisinin, V.V. Shcheglov, N.I. Kleymenov. Moscow, 2003. 456 p.

10. Radiobiologiya. Radiatsionnaya bezopasnost' sel'skokhozyaystvennykh zhivotnykh [Radiobiology. Radiation safety of agricultural animals]. Eds.: V.A. Budarkov, A.S. Zenkin. Moscow, Kolos Publ., 2008. 351 p.

11. Fiziologicheskie, biokhimicheskie i biometricheskie pokazateli normy eksperimental'nykh zhivotnykh. Spravochnik [Physiological, biochemical and biometric indices of the norms of experimental animals. Reference]. Saint Petersburg, «LEMA» Publ., 2013. 116 p.

12. Vasil'eva E.A. Klinicheskaya biokhimiya sel'skokhozyaystvennykh zhivotnykh [The clinical biochemistry of farm animals]. Moscow, Rossel'hozizdat, 1982. 254 p.

13. Gromyko E.V. Otsenka sostoyaniya organizma korov metodami biokhimii [Assessment of the body of cows by methods of biochemistry]. Ekologicheskiy vestnik Severnogo Kavkaza – Ecological Bulletin of the North Caucasus, 2005, no. 2, pp. 80-94.

14. Yakovenko A.M., Antonenko T.I., Selionova M.I. Biometricheskie metody analiza kachestvennykh i kolichestvennykh priznakov v zootekhnii. Uchebnoe posobie [Biometric methods of analysis of qualitative and quantitative traits in animal science. Textbook]. Eds.: A.M. Jakovenko, T.I. Antonenko, M.I. Selionov. Stavropol', Agrus Publ., 2013. 91 p.

15. GOST 33215-2014. Rukovodstvo po soderzhaniyu i ukhodu za laboratornymi zhivotnymi. Pravila oborudovaniya pomeshcheniy i organizatsii protsedur [The State Standart «Guidelines for accommodation and care of animals. Environment, housing and management»]. Moscow, «Standartinform», 2016. 14 p.

16. Gubareva O.S. Vliyanie vneshnego oblucheniya ovets na vosproizvodstvo i perevarimost' pitatel'nykh veshchestv pri ponizhennykh urovnyakh kormleniya [Effect of external exposure gamma-radiation and reduced feed intake on reproduction and digestibility in ewes]. Radiatsiya i risk – Radiation and Risk, 2012, vol. 21, no. 2, pp. 27-32.

17. Garashchenko S.V. Vliyanie kormovogo stressa pri polnom golodanii na vistseral'nye funktsii u sobak i korrektsiya stress-reaktsiy tsiklosilolom. Avtoref. diss. kand. med. nauk [The Influence of feed stress at full starvation on visceral function in dogs and correction of stress reactions by ziklosellol. Can. med. sci. diss.]. Tjumen', 2003. 16 p.

18. Ivanova V.F., Puzyrev A.A., Kostyukevich S.V., Dray R.V. Strukturnye izmeneniya v stenke kishki krys pri golodanii [Structural changes in the intestinal wall of rats under fasting]. Morfologiya – Morphology, 2009, vol. 136, no. 6, pp. 62-68.

19. Skverchinskaya E.A., Tavrovskaya T.V., Novozhilov A.V. Aktivnost' Na+, K+-ATFazy eritrotsitov krys pri prodolzhitel'nom alimentarnom golodanii [Activity of Na+, K+-ATPase of erythrocytes of rats during prolonged nutritional starvation]. Zhurnal evolyutsionnoy biokhimii i physiologii – Journal of Evolutionary Biochemistry and Physiology, 2013, vol. 49, no. 2, pp. 144-152.

20. Grigor'eva T.E., Yur'eva E.V., Ivanov G.I. Izofermentnyy sostav shchelochnoy fosfatazy syvorotki krovi krupnogo rogatogo skota v zavisimosti ot vozrasta i fiziologicheskogo sostoyaniya zhivotnykh [Isozyme composition of alkaline phosphatase of blood serum of cattle based on age and fisiological status of ani-mals]. Sel'skokhozyaystvennaya biologiya – Agricultural Biology, 1991, no. 4, pp. 40-43.

21. Willich N., Stamm A., Bogl W. Serum-amylase and other enzymes as indicatore radiation damage. BGA Schriften, 1986, vol. 2, pp. 181-183.

22. Ul'yanenko L.N. Biokhimicheskie aspekty i osnovy biologicheskoy indikatsii radiatsionnogo porazheniya sel'skokhozyaystvennykh zhivotnykh. Diss. dokt. biol. nauk [Biochemical aspects and fundamentals of biological indication of radiation injury of agricultural animals. Dr. biol. sci. diss.]. Obninsk, 1990. 410 p.

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