Photodynamic reparative regeneration of the endometrium in infertility due to chronic endometritis

«Radiation and Risk», 2020, vol. 29, No. 1, pp.154-161

DOI: 10.21870/0131-3878-2020-29-1-154-161

Authors

Katsalap S.N. – Sen. Researcher, C. Sc., Med. Contacts: 1A Lithuanian Boulevard, Moscow, 117593, Russia. Tel.: 8-909-675-80-93, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Serebrennikova K.G. – Supervisor of Obstetrics and Gynecology, MD, Prof.
Arutyunyan N.A. – Researcher, C. Sc., Med.
Alekhin A.I. – Deputy Chief Physician, MD, Prof. CCH of RAS

Central Clinical Hospital Russian Academy of Sciences, Moscow

Abstract

The goal of photodynamic therapy (PDT), carried out according to the classical protocol, is the devitalization of highly proliferative tissues. At present, PDT is used mainly in the treatment of certain localizations of malignant neoplasms. At the same time, the package of basic parameters and conditions necessary for the successful treatment of malignant tumors includes the dosage of the photosensitizer (1.0-1.5 mg/kg for E6 chlorin derivatives), the time interval necessary for the photosen-sitizer to accumulate in the tumor (2-3 hours) and laser energy density (not less than 150 J/cm²). Fundamentally different possibilities of the PDT method can be realized as a result of changing the parameters of laser radiation, dosage, and the method of introducing a photosensitizer. In particular, a decrease in the energy density in combination with a decrease in the power density index of the laser radiation makes it possible to obtain the photoregeneration effect of some tissues. The study examined the ability of the endometrium to reparative regeneration after intracavitary PDT in infertility due to chronic endometritis. As a photosensitizer, Photoditazine was used. PDT was performed in the second phase of the menstrual cycle using the balloon-type intrauterine fiber optic diffuser (KOVB-660). The results of histological and immunohistochemical studies indicate the restoration of the morphofunctional state of the endometrium up to 30 days after treatment. Photodynamic therapy with intracavitary technique of administering Photoditazine is a minimally invasive treatment method and can be used on an outpatient basis without anesthetics. The absence of complications and side effects with intracavitary PDT indicates the safety of the method.

Key words
photodynamic therapy, photosensitizer, photoregeneration, energy density, infertility, chronic endometritis, regeneration, endometrium, balloon-type intrauterine fiber optic diffuser, regenerative medicine.

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