Adenylate cyclase activity in sheep platelets following external whole-body gamma-irradiation with different doses

«Radiation and Risk», 2020, vol. 29, No. 4, pp.136-145

DOI: 10.21870/0131-3878-2020-29-4-136-145

Authors

Shevchenko T.S. – Head Specialist, C. Sc., Biol. Contacts: 109 km, Kievskoe Sh., Obninsk, Kaluga region, Russia, 249032. Tel.: 8(484)399-69-38; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Kobyalko V.O. – Head of Lab., C. Sc., Biol. RIARAE.

Russian Institute of Radiology and Agroecology, Obninsk

Abstract

The paper presents results of study of adenylate cyclase activity in the platelets after external whole-body gamma-irradiation of 32 tsigai rams (Ovis aries) with 2, 4 and 6 Gy, at the dose rate of 1 Gy/hour. The irradiation caused the development of acute radiation damage of a different degree of severity. To detect changes in the basal and prostoglandin E1 stimulated (PGE1-stimulated) adenylate cyclase activity thin-layer chromatography (TLC) was carried out during the first 30 days of the damage development. After external gamma-irradiation directly proportional effect of dose on changes in the basal adenylate cyclase activity in the platelets was registered on the 1 and the 7 days of the damage development, the changes in the PGE1-stimulated activity was registered on the 7 day. The study results allow making the suggestion that detected changes in the adenylate cyclase activity in the platelets of irradiated rams may be resulted from the modification of cytoplasmic cell membranes and impaired functioning of membrane-bound cAMP-sensitive signaling pathway. It may be also caused by new cells with qualitatively new properties released from the bone marrow megakaryocytes pool into the bloodstream.

Key words
external gamma-radiation, acute radiation damage, sheep, platelets, basal adenylate cyclase activity, prostaglandin E1-stimulated adenylate cyclase activity.

References

1. Avdonin P.V., Kozhevnikova L.M. Regulation of expression and functional activity of adenilate cуclase. Biologicheskie membrany – Biological Membranes, 2007, vol. 24, no. 1, pp. 4-31. (In Russian).

2. Whitefield J.F., Bounton A.L., Macmanus J.P., Korska M., Tsang B.K. The regulation of cell proliferation by calcium and cyclic AMP. Mol. Cell Biochem., 1979, vol. 27, pp. 155-179.

3. Green D.R. Overview: apoptotic signaling pathways in the immune system. Immunol. Rev., 2003, vol. 193, pp. 5-9.

4. Orlovskaya I.A., Kozlov V.A., Toporkova L.B. Intracellular signal systems in regulation of erythroid cell apopthosis. Immunologiya – Immunology, 2006, vol. 27, no. 5, pp. 312-316. (In Russian).

5. Shaturny V.I., Shakhidzhanov S.S., Sveshnikova A.N., Panteleev M.A. Activators, receptors and signal transduction pathways of blood platelets. Biomeditsinskaya khimiya – Biomedical Chemistry, 2014, vol. 60, no. 2, pp. 182-200. (In Russian).

6. Minetti G., Low P.S. Erythrocyte signal transduction pathways and activates adenylate cyclase in human erythrocyte membrane at physiological calcium plasma concentrations. Blood Cells Mol. Dis., 1997, vol. 263, pp. 223-228.

7. Muravyov A.V., Koshelev V.B., Fadyukova O.E., Tikhomirova I.A., Maimistova A.A., Bulaeva S.V. Role of adenylyl cyclase activation in changes of microrheological properties of red blood cell. Biologicheskie membrany – Biological Membranes, 2011, vol. 28, no. 3, pp. 174-180. (In Russian).

8. Kudryashov Yu.B. Radiation biophysics (ionizing radiation). Moscow, Fismatlit Publ., 2004. 448 p. (In Russian).

9. Chubanov V.S., Rogov Yu. I., Konoplya E.F., Sholuch M.V. Functional interaction of the components ade-nylate cyclase system in rat liver after prenatal exposure to gamma-radiation. Radiatsionnaya biologiya. Radi-oekologiya – Radiation Biology. Radioecology, 1999, vol. 39, no. 4, pp. 394-398. (In Russian).

10. Shchukin V.M. Metabolism of cyclic nucleotides in lymphoid organs of ruminants under total external and internal radiation exposure. Avtoref. Cand. Sci. biol. diss. Moscow, 2000. 18 p. (In Russian).

11. Shevchenko T.S., Konopleva I.V. Adenylate cyclase activity in lymphocytes and platelets in irradiated cat-tle. Sel'skokhoz. biologiya – Agricultural Biology, 2011, vol. 46, no. 2, pp. 63-67. (In Russian).

12. Shevchenko T.S., Kobyalko V.O. Activity of cAMP system in sheep lymphocytes and platelets exposed to external -radiation in vivo. Radiats. biologiya. Radioekologiya – Radiation Biology. Radioecology, 2015, vol. 55, no. 4, pp. 411-419. (In Russian).

13. Shevchenko T.S., Konopleva I.V. On the action of the external gamma-radiation on adenylate cyclase activity in the blood cells in sheep. Sel'skokhozyaistvennaya biologiya – Agricultural Biology, 2015, vol. 50, no. 4, pp. 495-502. (In Russian).

14. Shevchenko T.S. Dynamics of adenylate cyclase activity in cows lymphocytes under total external gamma-radiation exposure. Problemy biologii produktivnykh zhivotnykh – Problems of Productive Animal Biolo-gy, 2016, no. 3, pp. 65-73. (In Russian).

15. Shevchenko T.S., Kobyalko V.O. Estimation of the adenylate cyclase activity in lymphocytes of sheep exposed to different doses of total external gamma-radiation. Radiatsionnaya biologiya. Radioecologiya – Radi-ation Biology. Radioecology, 2017, vol. 57, no. 2, pp. 171-178. (In Russian).

16. Physiological, biochemical and biometric indicators of the norm of experimental animals. Handbook. St. Petersburg, LEMA, 2013. 116 p. (In Russian).

17. Shevchenko T.S. Isolation of cell populations from peripheral blood of farm animals. Sel'skokho-zyaistvennaya biologiya – Agricultural Biology, 2007, vol. 42, no. 6, pp. 123-126. (In Russian).

18. Shevchenko A.S. Activity of blood cell enzymes of cyclic adenosine monophosphate metabolism in sheep and horses. Sel'skokhozyaistvennaya biologiya – Agricultural Biology, 1988, no. 6, pp. 124-125. (In Russian).

19. Bourlakova E.B., Atkarskaya M.V., Phatkullina L.D., Andreev S.G. Radiation-induced changes in the structural state of human blood cell membranes. Radiatsionnaya Biologiya. Radioecologiya – Radiation Biology. Radioecology, 2014, vol. 54, no. 2, pp. 162-168. (In Russian).

20. Mazurov A.V. Physiology and pathology of platelets. Moscow, Literra, 2011. 480 p. (In Russian).

Full-text article (in Russian)