Investigation of the effects of ionizing radiation on radiation-induced bystander changes in the cells of the blood system of mice at the inter-organism level

«Radiation and Risk», 2023, vol. 32, No. 1, pp.48-60

DOI: 10.21870/0131-3878-2023-32-1-48-60

Authors

Kogarko I.N. – Lead. Researcher, MD
Petushkova V.V. – Lead. Specialist, C. Sc., Econ. Contacts: 4 Kosygin str., Moscow, 119991, Russia. Tel: +7(495) 939-72-73; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Kogarko B.S. – Sen. Researcher, C. Sc., Phys.-Math.
Neyfakh E.A. – Sen. Researcher, C. Sc., Biol.
Ktitorova O.V. – Researcher, C. Sc., Biol.
Ganeev I.I. – Research Engineer
Pelevina I.I. – D. Sc., Biol. FRCCP RAS.
Pryakhin E.A. – Head of Dep., D. Sc., Biol.
Andreev S.S. – Sen. Researcher, C. Sc., Biol. URCRM.
Kuzmina N.S. – Sen. Researcher, D. Sc., Biol. FRCCP RAS, VIGG.
Selivanova E.I. – Sen. Researcher, C. Sc., Biol. FRCCP RAS, A. Tsyb MRRC.
1 N.N. Semyonov Federal Research Center for Chemical Physics, RAS, Moscow
2 Urals Research Center of Radiation Medicine, FMBA RF, Chelyabinsk
3 Vavilov Institute of General Genetics, RAS, Moscow
4 A. Tsyb MRRC, Obninsk

Abstract

The study was aimed at the task to determine the radiation-induced “bystander effects” at the inter-organism level. In the experiment, irradiated and non-irradiated mice kept together were used. The mice were irradiated at a dose of 3 Gy at a research radiobiological gamma-ray facility with sources of 137Cs. The number of leukocytes and the relative number of lymphocytes in peripheral blood of mice were evaluated at 3, 7, 14, 30, 60 and 90 days after the irradiation. In unirradiated “bystander” mice kept with irradiated animals, both in cages without a septum and with a septum, a possible tendency to decrease the number of leukocytes compared to the same indicators in biological control was found. When analyzing the relative number of lymphocytes, a tendency to decrease in this indicator in “bystander” mice both when kept without a partition and with a partition was shown. In unirradiated bystander animals when kept with irradiated animals in a cage with a partition, a statistically significant decrease in the relative number of lymphocytes in peripheral blood was recorded: at the 3-rd day (t=2.13; p=0.047), at the 30-th day (t=2.94; p=0.01) and at the 90-th day (t=3.07; p=0.01) after irradiation, as well as when kept in the same cage with irradiated mice without a partition at the 60-th day (t=2.24; p=0.038) after the start of the experiment. This result can be considered as a radiation-induced “bystander effect” in unirradiated animals. In irradiated animals, which were kept together with unirradiated animals in the cage with a partition, a statistically significant increase in the relative number of lymphocytes was recorded at the 3-rd day (t=2.6; p=0.02), 14-th day (t=2.61; p=0.018) and at the 60-th day (t=2.38; p=0.03) after irradiation, so the radiation-induced “rescue effect” was obtained. Based on the data obtained in this experiment, it is assumed that the radiation-induced “bystander effect” may be reversed, that is, unirradiated organisms are able to reduce radiation-induced effects in irradiated individuals ‒ the “rescue effect”.

Key words
ionizing radiation, “non-targeted effects”, structural and functional disorders of the genome, functional changes in the blood system, radiation-induced “bystander effect”, radiation-induced “rescue effect”, lymphocytes, leukocytes, outbred female mice, inter-organism interactions, multivariate analysis of the data obtained.

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