Antitumor effect of photodynamic therapy with photosensitizer Photoran E6 on melanoma B-16 and response of mouse peripheral blood to PDT

«Radiation and Risk», 2022, vol. 31, No. 3, pp.77-86

DOI: 10.21870/0131-3878-2022-31-3-77-86

Authors

Abramova O.B. – Head of Lab., C. Sc., Biol. Contacts: 4 Korolyov str., Obninsk, Kaluga region, Russia, 249035. Tel.: + 7 910 510 08 62; е-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Drozhzhina V.V. – Researcher
Kozlovtseva E.A. – Researcher
Sivovolova T.P. – Researcher. A. Tsyb MRRC.
Kaprin A.D. – General Director, Academician of RAS, MD, Prof. NMRRC.
A. Tsyb MRRC, Obninsk
¹NMRRC, Moscow

Abstract

Photodynamic therapy (PDT) is the effective method for the total eradication of some types of solid malignant neoplasms. The paper presents results of research on the effectiveness of PDT of the melanoma B-16 with the intraperitoneally injected novel Russian photosensitizer (PS) "Photoran E6". To assess the response of melanoma to PDT, the following indicators were used: tumor growth inhi-bition index (TGI,%), tumor regression rate (%), absolute tumor growth rate (K) in mice with continued neoplasia growth. To assess the proportion of peripheral blood cells and their morphological characteristics the therapy efficiency integrated health indicators were used: increase in life expectancy (ILE,%) of mice under study in comparison with the control and the proportion of cured animals on the 90th day after PDT. The results of the study demonstrate high antitumor efficacy of Photoran E6 for PDT of mouse melanoma. The observed changes in hemogram parameters and in leukocyte indices also indicate the effectiveness of the therapy, changes in the control samples indicate the spread of the malignant process, proliferative processes, impaired normal regeneration of blood elements, severe intoxication that cause destruction and degeneration of blood cells and drastic changes in the course of immune response.

Key words
melanoma, photodynamic therapy, tumor regression, leukogram, integrated medical indicators, Photoran E6.

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