Issues
Linear threshold effects of gama-irradiation of the terrestrial mollusk F. fruticum M. of the third age group
«Radiation and Risk», 2023, vol. 32, No. 3, pp.84-96
DOI: 10.21870/0131-3878-2023-32-3-84-96
Authors
Lavrentyeva G.V. – Head of Dep., D. Sc., Biol. Bauman Moscow State Technical University. Contacts: 2, Bazhenova str., Kaluga, Kaluga region, Russia, 248000. Tel.: +7 961 122 71 06; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .Cherkasova E.E. – Teacher
Mirzeabasov O.A. – Associate Prof., C. Sc., Techn.
Shoshina R.R. – Associate Prof., D. Sc., Biol.
Synzynys B.I. – Prof., D. Sc., Biol. IATE of NRU MEPhI.
1 Bauman Moscow State Technical University (Kaluga Branch), Kaluga
2 Obninsk Institute for Nuclear Power Engineering, Obninsk
Abstract
The ecocentric strategy of radiation protection involves the expansion of experimental bases of radiation-induced effects in representatives of biota. In this article we studied the change in the relevant indicator (mortality) of the terrestrial mollusk F. fruticum M. after gama-irradiation with doses from 10 to 300 Gy. The terrestrial mollusk is not yet included in the list of reference species, but it meets the requirements of the ICRP for reference organisms. The aim of the work is to establish dose-effect relationships in the study of mortality of the terrestrial mollusk F. fruticum M. The third age group after acute gama-irradiation is used to expand databases on radio-induced effects of biota. The results of a study for mollusks of only the third age group are presented. This research is part of an experiment to identify age-related features of radiosensitivity of a representative of terrestrial malacofauna. The age of the mollusks was determined by the number of revolutions of the shell. The conditions for keeping animals in laboratory were selected in such a way that mortality in the control group remained at zero during the entire experiment. The duration of the experiment is 210 days. The LD50/60 index for the mollusk was estimated by the calculated probit analysis method using the least squares method. The LD50/60 is 141.4+-26.3 Gy. The change in the indicator was estimated every 30 days after irradiation. A linear threshold dependence was established for each time range, starting from 60 days after irradiation. It contains two threshold transitions to a new level of mortality and, accordingly, three dose ranges: a dose-independent range at low doses of radiation, a dose-dependent range with an increase in the dose of radiation and a dose-independent plateau when switching to the level of absolute lethality. Four dose ranges of mortality of terrestrial mollusks were established for 210 days after irradiation: 10-130 Gy (100% survival rate), 140-160 Gy (absolute mortality after 180 days), 170-190 Gy (absolute mortality after 150 days), 200-300 Gy (100% mortality after 90 days). At the same time, the greatest dynamics of the increase in mortality is observed in the first 60 days after irradiation.
Key words
ecocentric concept, reference species, relevant indicator, terrestrial mollusk, LD50/60, mortality, gama-irradiation, threshold effects, laboratory experiment, radiation-induced effect.
References
1. ICRP, 2008. Environmental protection: the concept and use of reference animals and plants. ICRP Publication 108. Ann. ICRP, 2008, vol. 38, no. 4-6, pp. 1-234.
2. ICRP, 2009. Environmental protection: transfer parameters for reference animals and plants. ICRP Publication 114. Ann. ICRP, 2009, vol. 39, no. 16, pp. 1-111.
3. ICRP, 2014. Protection of the environment under different exposure situations. ICRP Publication 124. Ann. ICRP, 2014, vol. 43, no. 1, pp. 1-59.
4. Lavrentyeva G., Shoshina R., Mirzeabasov O., Synzynys B. Influence of Sr-90 on the morphometric indices and the level of proteins of metallothioneins in the soft tissues of terrestrial mollusks Bradybaena fruticum in the area of location of the regional radioactive waste storage facility. Book of abstracts 2nd Int. Symp. “Physics, Engineering and Technologies for Biomedicine”. Moscow, MEPhI, 2017, pp. 246-247.
5. Gudkov D.I., Nazarov A.B., Dzyubenko E.V., Kaglyan A.E., Klenus V.G. Radioecological studies of fresh-water mollusks in the Chernobyl accident exclusion zone. Radiatsionnaya biologiya. Radioekologiya – Radiation Biology. Radioecology, 2009, vol. 49, no. 6, pp. 703-713. (In Russian).
6. Romanova E.M., Indiryakova O.A., Kuranova A.P. Prospects for the use of mollusks in the bioindication of water bodies. Izvestiya Orenburgskogo gosudarstvennogo agrarnogo universiteta. Biologicheskiye nauki – Proceedings of the Orenburg State Agrarian University. Biological sciences, 2008, vol. 4, no. 20, pp. 157-159. (In Russian).
7. Effects of radiation on the environment. UNSCEAR 2008. Report to the General Assembly with Scientific Annexes. Volume II, Scientific Annex E. Effect of ionizing radiation on non-human biota. New York, United Nations, 2011.
8. Lavrentyeva G.V., Shoshina R.R., Mirzeabasov O.A., Synzynys B.I. Impact of 90Sr on morphometric indi-ces and metallothioneins level in the soft tissues of Bradybaena fruticum inhabiting the regional radioactive wastes storage facility. Radiatsiya i risk – Radiation and Risk, 2017, vol. 26, no. 4, pp. 43-51. (In Russian).
9. Cherkasova E.E., Lavrentyeva G.V., Trofimova L.A. The effect of -irradiation on the biomass of the terres-trial mollusk Fruticicola Fruticum of different age groups. Rep. IVth Int. Scientific and Practical Conf. “Modern problems of radiobiology, radioecology and agroecology”. Obninsk, RIRAE, 2021, pp. 97-100. (In Russian).
10. Cherkasova E.E., Lavrentyeva G.V., Trofimova L.A. Effect of -irradiation on the relevant indicator of the terrestrial mollusk Fruticicola Fruticum of different age groups. Rep. Int. Scientific and Practical Conf. “Radio-ecological consequences of radiation accidents: on the 35th anniversary of the Chernobyl accident”. Obninsk, RIRAE, 2021, pp. 228-232. (In Russian).
11. Cherkasova E.E., Lavrentyeva G.V., Trofimova L.A. Substantiation of the age and conditions of the terrestrial mollusk Fr. fruticum for laboratory experiments. Abstracts XVth Int. Scientific and Practical Conf. “AtomFuture 2019”. Obninsk, MEPhI, 2020, pp. 18-19. (In Russian).
12. Petukhova G.A. Mollusks as sensitive test indicators of the state of the periphyton under the action of anthropogenic pollutants. Vestnik Tyumenskogo gosudarstvennogo universiteta – Bulletin of the Tyumen State University, 2005, no. 5, pp. 97-100. (In Russian).
13. Ryeznik E.P., Popov V.N. Perspective of snails (Helicidae) breeding in terms of farming in Crimea. Ekosistemy, ikh optimizatsiya i okhrana – Ecosystems, their Optimization and Protection, 2010, vol. 21, no. 2, pp. 153-157. (In Russian).
14. Shipunov A.B., Baldin E.M., Volkova P.A., Korobeynikov A.I., Nazarova S.A., Petrov S.V., Sufiyanov V.G. Naglyadnaya statistika. Ispol’zuyem R! [Visual statistics. Use R!]. Moscow, DMK Press, 2012. 298 p.
15. Cherkasova E.E., Lavrentyeva G.V., Synzynys B.I. Experimental observation of half lethal dose determinant for terrestrial mollusk Fruticicola Fruticum. Mezhdunarodnyy nauchno-issledovatel’skiy zhurnal – International Research Journal, 2020, vol. 119, no. 5, pp. 162-167. (In Russian).
16. Sarapultseva E.I., Malina Ju.Yu., Sarapultsev B.I. Linear threshold effects of Daphnia magna -irradiation at low doses. Radiatsiya i risk – Radiation and Risk, 2007, vol. 16, no. 2-4, pp. 33-38. (In Russian).
17. Skorobagat’ko D.A., Strashnyuk V.Yu., Mazilov A.A. Life expectancy in the offspring of Drosophila mela-nogaster after acute gamma irradiation. Vestnik Khar’kovskogo natsional’nogo universiteta imeni V.N. Karazina. Seriya: Biologiya – The Journal of V.N. Karazin Kharkiv National University. Series: Biology, 2016, no. 26, pp. 74-84. (In Russian).
18. Geras’kin S.A., Dikarev V.G., Udalova A.A., Dikareva N.S. Regularities of induction of cytogenetic damage in the root meristem of barley seedlings by small doses of ionizing radiation. Radiatsionnaya biologiya. Radioekologiya – Radiation Biology. Radioecology, 1999, vol. 39, no. 4, pp. 373-383. (In Russian).
19. Sarapultseva E.I., Gorski A.I., Malina J.J. Radiation risk of mortality and reduced life expectancy at daphnids gamma-irradiated at low doses. Radiatsiya i risk – Radiation and Risk, 2011, vol. 20, no. 1, pp. 34-40. (In Russian).
20. Cherkasova E.E., Lavrentyeva G.V., Synzynys B.I. Calculation of the half-year dose of the terrestrial mollusk Fruticicola Fruticum. Materials XXIInd Int. Conf “Sakharov readings 2022: environmental problems of the XXI century. Minsk, 2022, vol. 1, pp. 325-328. (In Russian).
21. Gudkov I.N., Kudyasheva A.G., Moskalev A.A. Radiobiologiya s osnovami radioekologii [Radiobiology with the basics of radioecology]. Syktyvkar, Syktyvkar State University, 2015. 512 p. (In Russian).
22. Lazarevich N.V., Sergeyeva I.I., Lazarevich S.S. Radiobiologiya: kurs lektsiy. Radiobiologiya zhivotnykh i cheloveka [Radiobiology: a course of lectures. Radiobiology of animals and humans]. Gorki, BGSHA, 2012. 103 p. (In Russian).
Full-text article (in Russian)