RADIATION & RISK
Bulletin of the National Radiation and Epidemiological Registry
since 1992

Changes in the cellular composition of blood in unirradiated and irradiated mice kept in contact at the inter-organismic level

«Radiation and Risk», 2023, vol. 32, No. 4, pp.147-161

DOI: 10.21870/0131-3878-2023-32-4-147-161

Authors

Kogarko I.N. – Head of Lab., Lead. Researcher, MD
Petushkova V.V. – Lead. Specialist, C. Sc., Econ. Contacts: 4 Kosygin str., Moscow, 119991, Russia. Tel: 8 (903) 295-11-33; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Kogarko B.S. – Sen. Researcher, C. Sc., Phys.-Math.
Neyfakh E.A. – Sen. Researcher, C. Sc., Biol.
Ktitorova O.V. – Researcher, C. Sc., Biol.
Ganeev I.I. – Research Engineer. FRCCP RAS.
Kuzmina N.S. – Sen. Researcher, D. Sc., Biol. FRCCP RAS, VIGG.
Selivanova E.I. – Sen. Researcher, C. Sc., Biol. FRCCP RAS, A. Tsyb MRRC.
1 N.N. Semyonov Federal Research Center for Chemical Physics, RAS, Moscow
2 Vavilov Institute of General Genetics, RAS, Moscow
3 A. Tsyb MRRC, Obninsk

Abstract

In the study conducted on outbred female mice, the radiation-induced “bystander effect” and the “rescue effect“ were found to be formed at an inter-organismic level. Unirradiated and gamma-irradiated (3 Gy) outbred white female mice were placed in one cage. The effect of co-residing was registered with the use of hemopoietic cells of peripheral blood by comparing to the population of hemopoietic cells in irradiated and unirradiated mice placed in different cages. The “bystander effect“ was observed in the monocytes subpopulation on the 3rd, 7th and 60th days. The effect was also observed in the neutrophils subpopulation on the 7th and 90th days. The “rescue effect” was observed in irradiated cells. The 5.8-fold (increase in eosinophils was registered on the 30th day (t=2.85; p=0.01), the 1.6-fold increase in eosinophils was observed on the 60th day, the 3-fold increase in eosinophils was registered on the 90th day (t=2.28; p=0.04). The effect of neutrophils subpopulation is slightly expressed, it can be considered as a tendency to increase the number of neutrophils on the 14th and 30th days. When the “rescue effect“ on irradiated mice was estimated with the use of monocytes the 5.7-fold increase was registered on the 30th day (t=2.94; p=0.01), the 7.3-fold increase was registered on the 90th day (t=2.41; p=0.03). According to the data obtained the “bystander effect” and the “rescue effect” are observed in all studied hemopoietic cells subpopulations, however they respond differently. The findings demonstrate the manifestation of the “bystander effect” and the “rescue effect” at the inter-organismic level. However, their intensity and trend depend to a large extent on the time period after irradiation, the contact type (tactile or olfactory) and a cellular subpopulation.

Key words
ionizing radiation, "nontargeted effects", structural and functional disorders of the genome, radiation-induced "bystander effect", radiation-induced "rescue effect", neutrophils, eosinophils, monocytes.

References

1. Nikitaki Z., Mavragani I.V., Laskaratou D.A., Gika V., Moskvin V.P., Theofilatos K., Vougas K., Stewart R.D., Georgakilas A.G. Systemic mechanisms and effects of ionizing radiation: a new 'old' paradigm of how the bystanders and distant can become the players. Semin. Cancer Biol., 2016, vol. 37-38, pp. 77-95.

2. Feng R.T., Weng K.L. Molecular mechanisms of low dose ionizing radiation-induced hormesis, adaptive responses, radioresistance, bystander effects, and genomic instability Int. J. Radiat. Biol, 2015, vol. 91, no. 1, pp. 13-27.

3. Matsumoto H., Takahashi A., Ohnishi T. Radiation-induced adaptive resp

onses and bystander effects. Biol. Sci. Space, 2004, vol. 18, no. 4, pp. 247-254.

4. Reis P., Lourenço J., Carvalho F.P., Oliveira J., Malta M., Mendo S., Pereira R. RIBE at an inter-organismic level: a study on genotoxic effects in Daphnia magna exposed to waterborne uranium and a uranium mine effluent. Aquat. Toxicol., 2018, vol. 198, pp. 206-214.

5. Mothersill C., Smith R.W., Saroya R., Denbeigh J., Rowe B., Banevicius L., Timmins R., Moccia R., Seymour C.B. Irradiation of rainbow trout at early life stages results in legacy effects in adults. Int. J. Radiat. Biol., 2010, vol. 86, no. 10, pp. 817-828.

6. Mothersill C., Smith R., Wang J., Rusin A., Fernandez-Palomo C., Fazzari J., Seymour C. Biological entanglement-like effect after communication of fish prior to X-ray exposure. Dose Response, 2018, vol. 16, no. 1, pp. 1559325817750067. DOI: 10.1177/1559325817750067.

7. Richard S., Wang J., Seymour C., Fernandez-Palomo C., Fazzari J., Schültke E., Bräuer-Krisch E., Laissue J., Schroll C., Mothersill C. Homogenous and microbeam X-ray radiation induces proteomic changes in the brains of irradiated rats and in the brains of nonirradiated cage mate rats. Dose Response, 2018, vol. 16, no. 1, pp. 1559325817750068. DOI: 10.1177/1559325817750068.

8. Directive 2010/63/EU of the European Parliament and of the Council of the European Union on the protection of animals used for scientific purposes. Saint Petersburg, Rus-LASA, 2012. (In Russian).

9. European Convention for the protection of vertebrate animals used for experiments or for other scientific purposes. Strasbourg, 1986. Available at: https://rm.coe.int/168007a6a8 (Accessed 15.05.2023). (In Russian).

10. Yu K.N. Radiation-induced rescue effect. J. Radiat. Res., 2019, vol. 60, no. 2, pp. 163-170.

11. Chen S., Zhao Y., Han W., Chiu S.K., Zhu L., Wu L., Yu K.N. Rescue effects in radiobiology: unirradiated bystander cells assist irradiated cells through intercellular signal feedback. Mutat. Res., 2011, vol. 706, pp. 59-64.

12. Fu J., Yuan D., Xiao L., Tu W., Dong C., Liu W., Shao C. The crosstalk between -irradiated Beas-2B cells and its bystander U937 cells through MAPK and NF-κB signaling pathways. Mutat. Res., 2016, vol. 783, pp. 1-8.

13. Fu J., Wang J., Wang X., Wang P., Xu J., Zhou C., Bai Y., Shao C. Signaling factors and pathways of α-particle irradiation induced bilateral bystander responses between Beas-2B and U937 cells. Mutat. Res., 2016, vol. 789, pp. 1-8.

14. Kogarko I.N., Petushkova V.V., Kogarko B.S., Pryakhin E.A., Neyfakh Е.А., Ktitorova O.V., Andreev S.S., Ganeev I.I., Kuzmina N.S., Selivanova E.I. Pelevina I.I. Investigation of the effects of ionizing radiation on radiation-induced bystander changes in the cells of the blood system of mice at the inter-organism level. Radiatsiya i risk – Radiation and Risk, 2023, vol. 32, no. 1, pp. 48-60. (In Russian).

15. Pelevina I.I., Kogarko I.N., Pryakhin E.A., Petushkova V.V., Kogarko B.S., Neyfakh Е.А., Andreev S.S., Ktitorova O.V., Ganeev I.I. Study of the disorders of the blood system of irradiated and unirradiated animals kept in contact. Radiatsionnaya biologiya. Radioekologiya – Radiation Biology. Radioecology, 2022, vol. 62, no. 5, pp. 477-483. (In Russian).

16. Petushkova V.V., Pelevina I.I., Kogarko I.N., Neyfakh E.А., Kogarko B.S., Ktitorova O.V. Radiation-induced inter-organism bystander effects. Some aspects of the transmission. Radiatsionnaya biologiya. Radioekologiya – Radiation Biology. Radioecology, 2020, vol. 60, no. 3, pp. 229-238. (In Russian).

17. Pelevina I.I., Akleev A.V., Kogarko I.N., Petushkova V.V., Kogarko B.S., Pryakhin E.A., Neyfakh Е.А., Ktitorova O.V. Radiation-chemical effects of ionizing radiation on the organism and genotoxic disorders of the blood system. Khimicheskaya fizika – Russian Journal of Physical Chemistry, 2021, vol. 40, no. 12, pp. 48-55. (In Russian).

18. Petushkova V.V., Pelevina I.I., Kogarko I.N., Neyfakh Е.А., Kogarko B.S., Ktitorova O.V. Radiation-induced inter-organism bystander effects. Some aspects of the transmission. Biol. Bull., 2020, vol. 47, no. 12, pp. 1610-1617.

19. Kogarko I.N., Petushkova V.V., Kogarko B.S., Pryakhin E.A., Neyfakh Е.А., Ktitorova O.V., Andreev S.S., Ganeev I.I., Kuzmina N.S., Selivanova E.I. Investigation of the role of contact effects of irradiated animals on unirradiated animals under the influence of radiation. Annual XXVII conference of the FRCCP RAS of the department of CIMBIO: abstracts of reports. Moscow, 2022, pp. 77-86. (In Russian).

20. Andreev S.S., Aldibekova A.E., Kogarko I.N., Petushkova B.C., Acleev A.C. New evidence of inter-organism bystander effects in mice. VIII Congress on Radiation Research: abstracts of reports. Dubna, 2021, pp. 83. (In Russian).

21. Surinov B.P., Isaeva V.G., Dukhova N.N. The changes in immunomodulatory and attractive properties of mice secretions after radiation exposure or induction of the “bystander effects”. Radiatsionnaya biologiya. Radioekologiya – Radiation Biology. Radioecology, 2021, vol. 61, no. 1, pp. 5-13. (In Russian).

22. Ermakov A.V., Konkova M.S., Kostyuk S.V., Veiko N.A. DNA signaling pathway that ensures the development of the radiation effect of the witness in human cells. Radiatsionnaya biologiya. Radioekologiya – Radiation Biology. Radioecology, 2011, vol. 51, no. 6, pp. 651-659. (In Russian).

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