Issues
The effect of multiple ionizing radiation on the activity of antioxidant enzymes of Escherichia coli ”PL-6” cells
«Radiation and Risk», 2024, vol. 33, No. 1, pp.68-76
DOI: 10.21870/0131-3878-2024-33-1-68-76
Authors
Gallyamova M.Yu. – Res. AssistantVagin K.N. – Lead. Researcher, Head of Lab., D. Sc., Biol., KFU. Contacts: Nauchnyj gorodok-2, Kazan, 420075, Republic of Tatarstan. Tel.: +7 (843) 239 53 20; e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. .
Gaynutdinov T.R. – Lead. Researcher, Head of Sec., C. Sc., Biol., KFU
Rakhmatullina G.I. – Sen. Research Officer, Head of Sec., C. Sc., Biol.
Ryzhkin S.A. – Sen. Research, Head of Dep., MD, Associate Prof., RMACPE MOH Russia, Kazan SMU MOH Russia, KFU. FCTRBS-ARRVI.
1 Federal Center for Toxicological, Radiation and Biological Safety, Kazan
2 Russian Medical Academy of Continuous Professional Education, Ministry of Healthcare of the Russian Federation, Moscow
3 Kazan State Medical University, Ministry of Healthcare of the Russian Federation, Kazan
4 Kazan (Volga region) Federal University, Kazan
Abstract
The article presents the analysis of enzymatic activity of Escherichia coli “PL-6” cells following multiple gamma irradiation with increasing doses. For irradiation the stationary gamma ray unit “Researcher” (60Co) with the dose rate of 36.7 Gy/min was used. Radiation doses to the bacterial cells increased from 3.5 to 10 kGy in 0.5 kGy increment, and from 10 to 30 kGy in 5 kGy increment. Unirradiated suspended bacterial cells were used as control. The irradiated cells enzymatic activity was estimated with the spectrophotometric method on the "MultiSkan Go" device (Thermo Fisher Scientific, Finland). The results of the study demonstrate the increase in the E. coli ”PL-6” cells radioresistance caused by gamma irradiation of the cells with gradually increased doses. Exposure of the bacteria to a higher gamma radiation dose caused decrease in the number of viable cells from 109 CFU/ml in the control culture sample to 102 CFU/ml after irradiation at a dose of 30 kGy. The most radiation-resistant cells were selected from cells undergone successive multiple irradiation with constantly increasing dose of ionizing radiation. The enzymes of the antioxidant system in irradiated cells exhibit increased specific activity as compared to the enzymes in control, unirradiated, cells. The activity of enzymes increases with an increase in the radiation dose. Catalase activity increased after multiple irradiation by 5 times and peroxidase activity increased by 4 times. To compare the results in the groups, the Student's t-test was applied at p<0.05.
Key words
ionizing radiation, 60Co, catalase, peroxidase, radioresistance, radiosensitivity of E. coli "PL6" cells, antioxidant system, gamma irradiation, enzyme synthesis, enzymatic activity, enzymes, radiobiology, environmental health.
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